Development of a Machine Learning-Based Cysticidal Assay and Identification of an Amebicidal and Cysticidal Marine Microbial Metabolite against Acanthamoeba
ABSTRACT Traditional cysticidal assays for Acanthamoeba species revolve around Desk treating cysts with compounds and manually observing the culture for evidence of excystation.This method is time-consuming, labor-intensive, and low throughput.We adapted and trained a YOLOv3 machine learning, object detection neural network to recognize Acanthamoeb